Journal: Viruses

Article Title: Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection.

doi: 10.3390/v11100935

Figure Lengend Snippet: Figure 3. Chromatin distribution and nuclear volume of infected Vero cells. (A) Confocal microscopy slices of unexpanded and expanded nuclei of non-infected and infected cells at 4, 8, 12, and 16 hpi. Chromatin was stained with a mixture of histone Abs (H3K27ac, H3K9me3, H4K20me3; cyan) and viral capsid protein with VP5 MAb (magenta). Scale bars are 5 µm. (B) A set of images showing a segmented nucleus and the positioning of its nuclear envelope (red) based on the chromatin staining.

Article Snippet: Cell samples were incubated with a primary mouse antibody against HSV-1 capsid protein VP5 (mAb 6F10, Santa Cruz Biotechnology Inc., Dallas, TX, USA), and with a mixture of primary rabbit antibodies against histone modifications H3K27ac (Abcam 4729, Abcam, Cambridge, UK), H3K9me3 (NB211073, Novus biological, Cambridge, UK) and H4K20me3 (Abcam 9053) to label chromatin.

Techniques: Infection, Confocal Microscopy, Staining

Journal: Viruses

Article Title: Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection.

doi: 10.3390/v11100935

Figure Lengend Snippet: Figure 4. Viral capsid and capsid protein localization in the nucleus. The distribution of the VP5 capsid protein and capsids as a function of the distance from the NE at various times post-infection. (A) A quantitative analysis of 3D confocal expansion microscopy images of infected cells (n = 9–10) showing the localization of VP5 as a function of the distance from the NE. (B) A TEM image of an infected cell at 16 hpi. The inset shows an enlarged view of the boxed area containing capsids in the proximity of the NE. Scale bar is 3 µm. (C) An analysis of TEM images illustrating the number of capsids as a function of the distance from the inner nuclear membrane (n = 10–13). The shaded areas around the lines represent the mean ± SEM.

Article Snippet: Cell samples were incubated with a primary mouse antibody against HSV-1 capsid protein VP5 (mAb 6F10, Santa Cruz Biotechnology Inc., Dallas, TX, USA), and with a mixture of primary rabbit antibodies against histone modifications H3K27ac (Abcam 4729, Abcam, Cambridge, UK), H3K9me3 (NB211073, Novus biological, Cambridge, UK) and H4K20me3 (Abcam 9053) to label chromatin.

Techniques: Infection, Microscopy, Membrane